Detection of Hepatozoon felis in Ticks Collected from Free-Ranging Amur Tigers (Panthera tigris altaica), Russian Far East, 2002–12

Thomas, LH, Seryodkin, IV, Goodrich, JM, Miquelle, DG, Birtles, RJ ORCID: https://orcid.org/0000-0002-4216-5044 and Lewis, JC 2016, 'Detection of Hepatozoon felis in Ticks Collected from Free-Ranging Amur Tigers (Panthera tigris altaica), Russian Far East, 2002–12' , Journal of Wildlife Diseases, 52 , pp. 674-676.

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Abstract

We collected 69 ticks from nine, free-ranging Amur tigers ( Panthera tigris altaica) between 2002 and 2011 and investigated them for tick-borne pathogens. DNA was extracted using alkaline digestion and PCR was performed to detect apicomplexan organisms. Partial 18S rDNA amplification products were obtained from 14 ticks from four tigers, of which 13 yielded unambiguous nucleotide sequence data. Comparative sequence analysis revealed all 13 partial 18S rDNA sequences were most similar to those belonging to strains of Hepatozoon felis (>564/572 base-pair identity, >99% sequence similarity). Although this tick-borne protozoon pathogen has been detected in wild felids from many parts of the world, this is the first record from the Russian Far East.

Item Type:	Article
Schools:	Schools > School of Environment and Life Sciences > Ecosystems and Environment Research Centre
Journal or Publication Title:	Journal of Wildlife Diseases
Publisher:	Wildlife Disease Association
ISSN:	0090-3558
Related URLs:	Publication
Funders:	Non funded research
Depositing User:	RJ Birtles
Date Deposited:	07 Nov 2016 16:23
Last Modified:	12 Nov 2019 14:06
URI:	http://usir.salford.ac.uk/id/eprint/40648

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