High-throughput phenotyping of uropathogenic E. coli isolates with Fourier transform infrared spectroscopy

AlRabiah, H, Correa, E ORCID: https://orcid.org/0000-0002-5122-4384, Upton, M and Goodacre, R 2013, 'High-throughput phenotyping of uropathogenic E. coli isolates with Fourier transform infrared spectroscopy' , The Analyst, 138 (5) , p. 1363.

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Fourier transform infrared (FT-IR) spectroscopy is an established rapid whole-organism fingerprinting method that generates metabolic fingerprints from bacteria that reflect the phenotype of the microorganism under investigation. However, whilst FT-IR spectroscopy is fast (typically 10 s to 1 min per sample), the approaches for microbial sample preparation can be time consuming as plate culture or shake flasks are used for growth of the organism. We report a new approach that allows micro-cultivation of bacteria from low volumes (typically 200 μL) to be coupled with FT-IR spectroscopy. This approach is fast and easy to perform and gives equivalent data to the lengthier and more expensive shake flask cultivations (sample volume = 20 mL). With this micro-culture approach we also demonstrate high reproducibility of the metabolic fingerprints. The approach allowed separation of different isolates of Escherichia coli involved in urinary tract infection, including members of the globally disseminated ST131 clone, with respect to both genotype and resistance or otherwise to the antibiotic Ciprofloxacin.

Item Type: Article
Schools: Schools > School of Computing, Science and Engineering > Salford Innovation Research Centre
Journal or Publication Title: The Analyst
Publisher: Royal Society of Chemistry
ISSN: 0003-2654
Related URLs:
Depositing User: Dr Elon Correa
Date Deposited: 10 Feb 2017 14:56
Last Modified: 27 Aug 2021 23:32
URI: http://usir.salford.ac.uk/id/eprint/41377

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