Correa, E 
ORCID: https://orcid.org/0000-0002-5122-4384 and Goodacre, R
2011,
'A genetic algorithm-Bayesian network approach for the analysis of metabolomics and spectroscopic data: application to the rapid detection of Bacillus spores and identification of Bacillus species'
, BMC Bioinformatics, 12 (1)
, p. 33.
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Abstract
Background The rapid identification of Bacillus spores and bacterial identification are paramount because of their implications in food poisoning, pathogenesis and their use as potential biowarfare agents. Many automated analytical techniques such as Curie-point pyrolysis mass spectrometry (Py-MS) have been used to identify bacterial spores giving use to large amounts of analytical data. This high number of features makes interpretation of the data extremely difficult We analysed Py-MS data from 36 different strains of aerobic endospore-forming bacteria encompassing seven different species. These bacteria were grown axenically on nutrient agar and vegetative biomass and spores were analyzed by Curie-point Py-MS. Results We develop a novel genetic algorithm-Bayesian network algorithm that accurately identifies sand selects a small subset of key relevant mass spectra (biomarkers) to be further analysed. Once identified, this subset of relevant biomarkers was then used to identify Bacillus spores successfully and to identify Bacillus species via a Bayesian network model specifically built for this reduced set of features. Conclusions This final compact Bayesian network classification model is parsimonious, computationally fast to run and its graphical visualization allows easy interpretation of the probabilistic relationships among selected biomarkers. In addition, we compare the features selected by the genetic algorithm-Bayesian network approach with the features selected by partial least squares-discriminant analysis (PLS-DA). The classification accuracy results show that the set of features selected by the GA-BN is far superior to PLS-DA.
| Item Type: | Article |
|---|---|
| Schools: | Schools > School of Computing, Science and Engineering > Salford Innovation Research Centre |
| Journal or Publication Title: | BMC Bioinformatics |
| Publisher: | BioMed Central |
| ISSN: | 1471-2105 |
| Related URLs: | |
| Depositing User: | Dr Elon Correa |
| Date Deposited: | 10 Feb 2017 15:07 |
| Last Modified: | 16 Feb 2022 18:11 |
| URI: | https://usir.salford.ac.uk/id/eprint/41380 |
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