Assessment of the efficacy of BTI Larvicide, VectoBac 12AS® (BTI AM65-52) against “nuisance” flies at water treatment plants in North West England

Wenjere, S 2017, Assessment of the efficacy of BTI Larvicide, VectoBac 12AS® (BTI AM65-52) against “nuisance” flies at water treatment plants in North West England , MSc by research thesis, University of Salford.

PDF (Thesis: MSc (by Research) in Biological Sciences) - Submitted Version
Download (3MB) | Preview


In treating wastewater using percolating filters, nature employs diverse biota, which includes nematoceran larvae, to sustain filter function. This study sought to generate evidence on the efficacy of a Bacillus thuringiensis var. israelensis-derived larvicide widely used in England for the control of adult flies that emerge. This was in part-fulfilment of the European Union’s new biocidal products licencing requirements.

Adult and larval fly counts of four species of “nuisance flies”, Limnophyes minimus, Metriocnemus eurynotus, Psychoda alternata and Sylvicola fenestralis were monitored at two wastewater treatment works (WWTW) in North West of England. At selected points relative to peak fly presence, selected filters were treated with the larvicide at concentrations of 80mg/L (low dose), 160 mg/L (medium dose) or 230 mg/L (high dose). Fly reduction and effectiveness of different doses were determined based on pre- and post-treatment counts.

A single treatment against S. fenestralis using the medium (recommended) dose achieved 100% larval and adult fly reductions within two and fourteen days respectively. Two treatments against P. alternata at the low (recommended) dose achieved 93% and 95% larval and adult reductions overall, 48 hours and 23 days after the second treatment respectively. Forty-eight hours and one month after second treatment, larval and adult L. minimus respectively showed 100% and 30% overall reductions at low dose, 100% and 91% at medium (recommended) dose and, 100% and 90% at high dose. At similar post-treatment points, larval and adult M. eurynotus showed 100% and 94% overall reductions respectively at low dose, 97% and 99% at medium (recommended) dose and 100% across both larvae and adults at high dose.

The biolarvicide showed no effect against non-target organisms but was harmful and effective against target-species. Reduced and increased treatment strengths trialled against chironomid species showed slightly reduced efficacy and no added benefit respectively.

Item Type: Thesis (MSc by research)
Schools: Schools > School of Environment and Life Sciences > Biomedical Research Centre
Funders: Resource Chemical Ltd
Depositing User: Standreck Wenjere
Date Deposited: 19 Feb 2018 13:52
Last Modified: 21 Dec 2021 13:25

Actions (login required)

Edit record (repository staff only) Edit record (repository staff only)