Skin swabs with FTA® cards as a dry storage source for amphibian DNA

Ward, A, Hide, G ORCID: https://orcid.org/0000-0002-3608-0175 and Jehle, R ORCID: https://orcid.org/0000-0003-0545-5664 2019, 'Skin swabs with FTA® cards as a dry storage source for amphibian DNA' , Conservation Genetics Resources, 11 , pp. 309-311.

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Abstract

Amphibians are the most endangered group of vertebrates, and conservation measures increasingly rely on information drawn from genetic markers. The present study explores skin swabs with Whatman FTA® cards as a method to retrieve PCR-amplifiable amphibian DNA. Swabs from ten adult great crested newts (Triturus cristatus) were used to compare FTA® card-based protocols with tissue sampling based on toe clips. PCR success rates were measured for seven microsatellite markers and one mtDNA marker (ND4) after 6 months of sample storage. We demonstrate that the merging of eight FTA® card punches from Qiagen-based DNA extraction always led to successful amplifications in at least one replicate, at an overall PCR success rate of 78%. The newly established protocol has the potential for wide application to future DNA-based amphibian studies.

Item Type:	Article
Schools:	Schools > School of Environment and Life Sciences > Ecosystems and Environment Research Centre
Journal or Publication Title:	Conservation Genetics Resources
Publisher:	Springer
ISSN:	1877-7252
Related URLs:	Publication
Depositing User:	R Jehle
Date Deposited:	05 Mar 2018 13:39
Last Modified:	13 Oct 2020 13:00
URI:	http://usir.salford.ac.uk/id/eprint/46166

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