Ward, A, Hide, G ORCID: https://orcid.org/0000-0002-3608-0175 and Jehle, R
ORCID: https://orcid.org/0000-0003-0545-5664
2019,
'Skin swabs with FTA® cards as a dry storage source for amphibian DNA'
, Conservation Genetics Resources, 11
, pp. 309-311.
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Abstract
Amphibians are the most endangered group of vertebrates, and conservation measures increasingly rely on information drawn from genetic markers. The present study explores skin swabs with Whatman FTA® cards as a method to retrieve PCR-amplifiable amphibian DNA. Swabs from ten adult great crested newts (Triturus cristatus) were used to compare FTA® card-based protocols with tissue sampling based on toe clips. PCR success rates were measured for seven microsatellite markers and one mtDNA marker (ND4) after 6 months of sample storage. We demonstrate that the merging of eight FTA® card punches from Qiagen-based DNA extraction always led to successful amplifications in at least one replicate, at an overall PCR success rate of 78%. The newly established protocol has the potential for wide application to future DNA-based amphibian studies.
Item Type: | Article |
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Schools: | Schools > School of Environment and Life Sciences > Ecosystems and Environment Research Centre |
Journal or Publication Title: | Conservation Genetics Resources |
Publisher: | Springer |
ISSN: | 1877-7252 |
Related URLs: | |
Depositing User: | R Jehle |
Date Deposited: | 05 Mar 2018 13:39 |
Last Modified: | 15 Feb 2022 22:55 |
URI: | http://usir.salford.ac.uk/id/eprint/46166 |
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