Echinococcus granulosus cyst fluid enhances epithelial - mesenchymal transition

Mohammed, AA, Allen, JT and Rogan, MT ORCID: https://orcid.org/0000-0001-5399-8570 2018, 'Echinococcus granulosus cyst fluid enhances epithelial - mesenchymal transition' , Parasite Immunology, 40 (6) , e12533.

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Abstract

Aims
Cystic Echinococcosis is characterised by fluid filled hydatid cysts in the liver and lungs. The cysts are surrounded by a host fibrous layer (the pericyst) which acts to isolate the parasite from surrounding tissues. Previous studies in liver cysts have indicated that the parasite may be stimulating fibrosis. The aim of this study was to investigate whether Hydatid Cyst Fluid (HCF) could influence the potential for fibrosis to occur in lung tissue by stimulating epithelial to mesenchymal transition (EMT) in a human lung epithelial cell line.

Methods and Results
An adenocarcinoma-derived alveolar basal epithelial cell line (A549) was used as a model for human alveolar epithelial cells (AEC II). These were cultured in vitro with HCF (UK sheep origin). Assays to investigate cell proliferation, cell migration and expression of cytoskeletal markers showed that HCF could stimulate changes indicative of EMT, including enhanced cell proliferation and migration; increased expression of mesenchymal cytoskeletal markers (fibronectin and vimentin) accompanied by a down regulation of an epithelial marker (E-cadherin).

Conclusions
Molecules within hydatid cyst fluid are capable of inducing phenotypic changes in A549 cells indicating that the parasite has the potential to modify lung epithelial cells which could contribute to fibrotic reactions.

Item Type: Article
Schools: Schools > School of Environment and Life Sciences > Biomedical Research Centre
Journal or Publication Title: Parasite Immunology
Publisher: Wiley
ISSN: 1365-3024
Related URLs:
Depositing User: MT Rogan
Date Deposited: 02 May 2018 12:01
Last Modified: 02 May 2019 08:00
URI: http://usir.salford.ac.uk/id/eprint/46917

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