Tunicamycin induced endoplasmatic reticulum changes in endothelial cells investigated in vitro by confocal Raman imaging

Bik, E, Mielniczek, N, Jarosz, M, Denbigh, JL ORCID: https://orcid.org/0000-0002-6616-7757, Budzynska, R, Baranska, M and Majzner, K 2019, 'Tunicamycin induced endoplasmatic reticulum changes in endothelial cells investigated in vitro by confocal Raman imaging' , Analyst, 144 (22) , pp. 6561-6569.

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Abstract

This paper describes how tunicamycin (Tu), the most widely used pharmacological agent for inducing endoplasmic reticulum (ER) stress, interacts with endothelial cells. Our results show that tunicamycin enters the cells and accumulates within the ER area. ER stress takes place when improperly folded or damaged proteins begin to accumulate; however, spectroscopic markers of these changes have not been identified as yet. In this work, Raman spectroscopy and scanning electron microscopy imaging of individual endothelial cells treated with Tu were performed. The changes in the biochemical composition of endothelial cells induced by Tu attributed to ER stress were studied in detail. A main feature of the Tu impact on the cells was a decrease of the phospholipid content in the area of ER, and the most abundant lipid with phosphorus groups found there, was identified as sphingomyelin.

Item Type:	Article
Schools:	Schools > School of Environment and Life Sciences > Biomedical Research Centre
Journal or Publication Title:	Analyst
Publisher:	Royal Society of Chemistry
ISSN:	0003-2654
Related URLs:	Publication
Funders:	Ministry of Science and Higer Education, National Science Center
Depositing User:	JL Denbigh
Date Deposited:	26 Nov 2019 13:14
Last Modified:	17 Dec 2019 14:30
URI:	http://usir.salford.ac.uk/id/eprint/53172

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