Quantum dot-based thermal spectroscopy and imaging of optically trapped microspheres and single cells

Martinez Maestro, L ORCID: https://orcid.org/0000-0001-6629-5313, Haro Gonzalez, P, Ramsay, WT, del Rosal, B, Santacruz-Gomez, K, Iglesias de la Cruz, MC, Sanz Rodríguez, F, Chooi, JY, Rodriguez Sevilla, P, Bettinelli, M, Choudhury, D, Kar, JK, Garcia Sole, J, Jaque, D and Paterson, L 2013, 'Quantum dot-based thermal spectroscopy and imaging of optically trapped microspheres and single cells' , Small, 9 (12) , pp. 2162-2170.

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Laser‐induced thermal effects in optically trapped microspheres and single cells are investigated by quantum dot luminescence thermometry. Thermal spectroscopy has revealed a non‐localized temperature distribution around the trap that extends over tens of micrometers, in agreement with previous theoretical models besides identifying water absorption as the most important heating source. The experimental results of thermal loading at a variety of wavelengths reveal that an optimum trapping wavelength exists for biological applications close to 820 nm. This is corroborated by a simultaneous analysis of the spectral dependence of cellular heating and damage in human lymphocytes during optical trapping. This quantum dot luminescence thermometry demonstrates that optical trapping with 820 nm laser radiation produces minimum intracellular heating, well below the cytotoxic level (43 °C), thus, avoiding cell damage.

Item Type: Article
Schools: Schools > School of Computing, Science and Engineering
Journal or Publication Title: Small
Publisher: John Wiley & Sons, Inc.
ISSN: 1613-6810
Related URLs:
Funders: Universidad Autónoma de Madrid, Comunidad Autonoma de Madrid, Spanish Ministerio de Educacion y Ciencia, Caja Madrid Foundation, Fondazione Cariverona, fundacion Dr. Manuel Morales, Banco Santander and CEAL-UAM, UK Engineering and Physical Sciences Research Council (EPSRC), Heriot Watt University Life Science Interface Theme Scholarship
Depositing User: L Martinez Maestro
Date Deposited: 10 Dec 2019 10:25
Last Modified: 27 Aug 2021 21:32
URI: http://usir.salford.ac.uk/id/eprint/53264

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