Heat in optical tweezers

Martinez Maestro, L ORCID: https://orcid.org/0000-0001-6629-5313, del Rosal, B, Haro-Gonzalez, P, Ramsay, WT, Santacruz-Gomez, K, Iglesias-de la Cruz, MC, Sanz-Rodriguez, F, Chooi, JY, Rodriguez Sevilla, P, Choudhury, D, Kar, AK, Garcia Sole, J, Paterson, L and Jaque, D 2013, Heat in optical tweezers , in: SPIE NanoScience + Engineering, 2013, August 25 - 29, 2013, San Diego Convention Center.

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Abstract

Laser-induced thermal effects in optically trapped microspheres and single cells have been investigated by Luminescence Thermometry. Thermal spectroscopy has revealed a non-localized temperature distribution around the trap that extends over tens of microns, in agreement with previous theoretical models. Solvent absorption has been identified as the key parameter to determine laser-induced heating, which can be reduced by establishing a continuous fluid flow of the sample. Our experimental results of thermal loading at a variety of wavelengths reveal that an optimum trapping wavelength exists for biological applications close to 820 nm. This has been corroborated by a simultaneous analysis of the spectral dependence of cellular heating and damage in human lymphocytes during optical trapping. Minimum intracellular heating, well below the cytotoxic level (43 °C), has been demonstrated to occur for optical trapping with 820 nm laser radiation, thus avoiding cell damage.

Item Type: Conference or Workshop Item (Paper)
Schools: Schools > School of Computing, Science and Engineering
Journal or Publication Title: Proceedings of SPIE
Publisher: SPIE
ISBN: 9780819496607
ISSN: 0277-786X
Related URLs:
Depositing User: L Martinez Maestro
Date Deposited: 05 Dec 2019 10:44
Last Modified: 05 Dec 2019 10:45
URI: http://usir.salford.ac.uk/id/eprint/53349

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