Temperature sensing using fluorescent nanothermometers

Martinez Maestro, L ORCID: https://orcid.org/0000-0001-6629-5313, Vetrone, F, Naccache, R, Zamarron, A, Juarranz de la Fuente, A, Sanz-Rodriguez, F, Martin Rodriguez, E, Jaque, D, García Sole, J and Capobianco, J A 2010, 'Temperature sensing using fluorescent nanothermometers' , ACS Nano, 4 (6) , pp. 3254-3258.

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Abstract

Acquiring the temperature of a single living cell is not a trivial task. In this paper, we devise a novel nanothermometer, capable of accurately determining the temperature of solutions as well as biological systems such as HeLa cancer cells. The nanothermometer is based on the temperature-sensitive fluorescence of NaYF4:Er3+,Yb3+ nanoparticles, where the intensity ratio of the green fluorescence bands of the Er3+ dopant ions (2H11/2 → 4I15/2 and 4S3/2 → 4I15/2) changes with temperature. The nanothermometers were first used to obtain thermal profiles created when heating a colloidal solution of NaYF4:Er3+,Yb3+ nanoparticles in water using a pump−probe experiment. Following incubation of the nanoparticles with HeLa cervical cancer cells and their subsequent uptake, the fluorescent nanothermometers measured the internal temperature of the living cell from 25 °C to its thermally induced death at 45 °C.

Item Type:	Article
Schools:	Schools > School of Computing, Science and Engineering
Journal or Publication Title:	ACS Nano
Publisher:	ACS Publications
ISSN:	1936-0851
Related URLs:	Publication
Funders:	Universidad Autonoma de Madrid, Comunidad Autonoma de Madrid, Spanish Ministerio de Educacion y Ciencia, Banco Santander-CEAL-UAM, Natural Sciences and Engineering Research Council (NSERC) of Canada, Gouvernement du Que´bec, Ministe`re du De´veloppement e´conomique, de l’Innovation et de l’Exportation, Spanish Minsterio de Educacio´n
Depositing User:	L Martinez Maestro
Date Deposited:	03 Dec 2019 16:39
Last Modified:	27 Aug 2021 21:32
URI:	https://usir.salford.ac.uk/id/eprint/53363

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