Fusion transcripts in normal human cortex increase with age and show distinct genomic features for single cells and tissues

Mehani, B, Narta, K, Paul, D, Raj, A, Kumar, D, Sharma, A, Kaurani, L, Nayak, S, Dash, D, Suri, A, Sarkar, C and Mukhopadhyay, A ORCID: https://orcid.org/0000-0002-1089-7179 2019, 'Fusion transcripts in normal human cortex increase with age and show distinct genomic features for single cells and tissues' , Scientific Reports . (In Press)

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Abstract

Fusion transcripts can contribute to diversity of molecular networks in the human cortex. In this study, we explored the occurrence of fusion transcripts in normal human cortex along with single neurons and astrocytes. We identified 1305 non-redundant fusion events from 388 transcriptomes representing 59 human cortices and 329 single cells. Our results indicate while the majority of fusion transcripts in human cortex are intra-chromosomal (85%), events found in single neurons and astrocytes were primarily inter-chromosomal (80%). The number of fusions in single neurons was significantly higher than that in single astrocytes (p <0.05), indicating fusion as a possible contributor towards transcriptome diversity in neuronal cells. The identified fusions were largely private and 4 specific recurring events were found both in cortex and in single neurons but not in astrocytes. We found a significant increase in the number of fusion transcripts in human brain with increasing age both in single cells and whole cortex (p <0.0005 and <0.005, respectively). This is likely one of the many possible contributors for the inherent plasticity of the adult brain. The fusion transcripts in fetal brain were enriched for genes for long-term depression; while those in adult brain involved genes enriched for long-term potentiation pathways. Our findings demonstrate fusion transcripts are naturally occurring phenomenon spanning across the health-disease continuum, and likely contribute to the diverse molecular network of human brain.

Item Type: Article
Schools: Schools > School of Environment and Life Sciences > Biomedical Research Centre
Journal or Publication Title: Scientific Reports
Publisher: Nature Publishing Group
ISSN: 2045-2322
Depositing User: USIR Admin
Date Deposited: 08 Jan 2020 13:47
Last Modified: 08 Jan 2020 14:00
URI: http://usir.salford.ac.uk/id/eprint/56179

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