Fatty-acid uptake in prostate cancer cells using dynamic microfluidic raman technology

Tang, N-T, D. Snook, R, Brown, MD ORCID: https://orcid.org/0000-0003-1322-4671, Haines, BA, Ridley, A, Gardner, P and Denbigh, JL ORCID: https://orcid.org/0000-0002-6616-7757 2020, 'Fatty-acid uptake in prostate cancer cells using dynamic microfluidic raman technology' , Molecules, 25 (7) , e1652.

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Abstract

It is known that intake of dietary fatty acid (FA) is strongly correlated with prostate cancer progression but is highly dependent on the type of FAs. High levels of palmitic acid (PA) or arachidonic acid (AA) can stimulate the progression of cancer. In this study, a unique experimental set-up consisting of a Raman microscope, coupled with a commercial shear-flow microfluidic system is used to monitor fatty acid uptake by prostate cancer (PC-3) cells in real-time at the single cell level. Uptake of deuterated PA, deuterated AA, and the omega-3 fatty acids docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) were monitored using this new system, while complementary flow cytometry experiments using Nile red staining, were also conducted for the validation of the cellular lipid uptake. Using this novel experimental system, we show that DHA and EPA have inhibitory effects on the uptake of PA and AA by PC-3 cells.

Item Type: Article
Additional Information: ** From MDPI via Jisc Publications Router ** Licence for this article: https://creativecommons.org/licenses/by/4.0/ **Journal IDs: eissn 1420-3049 **History: published 03-04-2020; accepted 01-04-2020
Schools: Schools > School of Environment and Life Sciences > Biomedical Research Centre
Journal or Publication Title: Molecules
Publisher: MDPI
ISSN: 1420-3049
Related URLs:
SWORD Depositor: Publications Router
Depositing User: Publications Router
Date Deposited: 06 Apr 2020 11:55
Last Modified: 06 Apr 2020 12:00
URI: http://usir.salford.ac.uk/id/eprint/56772

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