Development of polymeric nanocomposite (Xyloglucan-co-Methacrylic acid/Hydroxyapatite/SiO 2 ) scaffold for bone tissue engineering applications—In-vitro antibacterial, cytotoxicity and cell culture evaluation

Aslam Khan, MU ORCID: https://orcid.org/0000-0002-2724-1336, Mehboob, H ORCID: https://orcid.org/0000-0003-1184-1326, Abd Razak, SI, Yahya, MY, Mohd Yusof, AH, Ramlee, MH ORCID: https://orcid.org/0000-0003-2705-8379, Sahaya Anand, TJ, Hassan, R, Aziz, A ORCID: https://orcid.org/0000-0002-8774-0348 and Amin, R 2020, 'Development of polymeric nanocomposite (Xyloglucan-co-Methacrylic acid/Hydroxyapatite/SiO 2 ) scaffold for bone tissue engineering applications—In-vitro antibacterial, cytotoxicity and cell culture evaluation' , Polymers, 12 (6) , e1238.

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Abstract

Advancement and innovation in bone regeneration, specifically polymeric composite scaffolds, are of high significance for the treatment of bone defects. Xyloglucan (XG) is a polysaccharide biopolymer having a wide variety of regenerative tissue therapeutic applications due to its biocompatibility, in-vitro degradation and cytocompatibility. Current research is focused on the fabrication of polymeric bioactive scaffolds by freeze drying method for nanocomposite materials. The nanocomposite materials have been synthesized from free radical polymerization using n-SiO2 and n-HAp XG and Methacrylic acid (MAAc). Functional group analysis, crystallinity and surface morphology were investigated by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction analysis (XRD) and scanning electron microscopy (SEM) techniques, respectively. These bioactive polymeric scaffolds presented interconnected and well-organized porous morphology, controlled precisely by substantial ratios of n-SiO2. The swelling analysis was also performed in different media at varying temperatures (27, 37 and 47 °C) and the mechanical behavior of the dried scaffolds is also investigated. Antibacterial activities of these scaffolds were conducted against pathogenic gram-positive and gram-negative bacteria. Besides, the biological behavior of these scaffolds was evaluated by the Neutral Red dye assay against the MC3T3-E1 cell line. The scaffolds showed interesting properties for bone tissue engineering, including porosity with substantial mechanical strength, biodegradability, biocompatibility and cytocompatibility behavior. The reported polymeric bioactive scaffolds can be aspirant biomaterials for bone tissue engineering to regenerate defecated bone.

Item Type: Article
Additional Information: ** From MDPI via Jisc Publications Router ** Licence for this article: https://creativecommons.org/licenses/by/4.0/ **Journal IDs: eissn 2073-4360 **History: published 29-05-2020; accepted 26-05-2020
Uncontrolled Keywords: antibacterial active, biocompatibility, nanotechnology, nanocomposite scaffolds, bone tissue engineering
Schools: Schools > School of Environment and Life Sciences
Journal or Publication Title: Polymers
Publisher: MDPI
ISSN: 2073-4360
Related URLs:
Funders: Universiti Teknologi Malaysia
SWORD Depositor: Publications Router
Depositing User: Publications Router
Date Deposited: 01 Jun 2020 10:49
Last Modified: 01 Jun 2020 11:00
URI: http://usir.salford.ac.uk/id/eprint/57134

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