Paul, D, Ansari, AH, Lal, M and Mukhopadhyay, A 
ORCID: https://orcid.org/0000-0002-1089-7179
2020,
'Human brain shows recurrent non-canonical microRNA editing events enriched for seed sequence with possible functional consequence'
, Non-Coding RNA, 6 (2)
, e21.
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Abstract
RNA editing is a post-transcriptional modification, which can provide tissue-specific functions not encoded in DNA. Adenosine-to-inosine is the predominant editing event and, along with cytosine-to-uracil changes, constitutes canonical editing. The rest is non-canonical editing. In this study, we have analysed non-canonical editing of microRNAs in the human brain. We have performed massively parallel small RNA sequencing of frontal cortex (FC) and corpus callosum (CC) pairs from nine normal individuals (post-mortem). We found 113 and 90 unique non-canonical editing events in FC and CC samples, respectively. More than 70% of events were in the miRNA seed sequence—implicating an altered set of target mRNAs and possibly resulting in a functional consequence. Up to 15% of these events were recurring and found in at least three samples, also supporting the biological relevance of such variations. Two specific sequence variations, C-to-A and G-to-U, accounted for over 80% of non-canonical miRNA editing events—and revealed preferred sequence motifs. Our study is one of the first reporting non-canonical editing in miRNAs in the human brain. Our results implicate miRNA non-canonical editing as one of the contributing factors towards transcriptomic diversity in the human brain.
| Item Type: | Article |
|---|---|
| Additional Information: | ** From MDPI via Jisc Publications Router ** Licence for this article: https://creativecommons.org/licenses/by/4.0/ **Journal IDs: eissn 2311-553X **History: published 02-06-2020; accepted 27-05-2020 |
| Schools: | Schools > School of Environment and Life Sciences |
| Journal or Publication Title: | Non-Coding RNA |
| Publisher: | MDPI |
| ISSN: | 2311-553X |
| Related URLs: | |
| Funders: | Council of Scientific and Industrial Research (CSIR), Government of India |
| SWORD Depositor: | Publications Router |
| Depositing User: | Publications Router |
| Date Deposited: | 04 Jun 2020 14:14 |
| Last Modified: | 16 Feb 2022 04:46 |
| URI: | https://usir.salford.ac.uk/id/eprint/57167 |
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