Human brain shows recurrent non-canonical microRNA editing events enriched for seed sequence with possible functional consequence

Paul, D, Ansari, AH, Lal, M and Mukhopadhyay, A ORCID: https://orcid.org/0000-0002-1089-7179 2020, 'Human brain shows recurrent non-canonical microRNA editing events enriched for seed sequence with possible functional consequence' , Non-Coding RNA, 6 (2) , e21.

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Abstract

RNA editing is a post-transcriptional modification, which can provide tissue-specific functions not encoded in DNA. Adenosine-to-inosine is the predominant editing event and, along with cytosine-to-uracil changes, constitutes canonical editing. The rest is non-canonical editing. In this study, we have analysed non-canonical editing of microRNAs in the human brain. We have performed massively parallel small RNA sequencing of frontal cortex (FC) and corpus callosum (CC) pairs from nine normal individuals (post-mortem). We found 113 and 90 unique non-canonical editing events in FC and CC samples, respectively. More than 70% of events were in the miRNA seed sequence—implicating an altered set of target mRNAs and possibly resulting in a functional consequence. Up to 15% of these events were recurring and found in at least three samples, also supporting the biological relevance of such variations. Two specific sequence variations, C-to-A and G-to-U, accounted for over 80% of non-canonical miRNA editing events—and revealed preferred sequence motifs. Our study is one of the first reporting non-canonical editing in miRNAs in the human brain. Our results implicate miRNA non-canonical editing as one of the contributing factors towards transcriptomic diversity in the human brain.

Item Type: Article
Additional Information: ** From MDPI via Jisc Publications Router ** Licence for this article: https://creativecommons.org/licenses/by/4.0/ **Journal IDs: eissn 2311-553X **History: published 02-06-2020; accepted 27-05-2020
Schools: Schools > School of Environment and Life Sciences
Journal or Publication Title: Non-Coding RNA
Publisher: MDPI
ISSN: 2311-553X
Related URLs:
Funders: Council of Scientific and Industrial Research (CSIR), Government of India
SWORD Depositor: Publications Router
Depositing User: Publications Router
Date Deposited: 04 Jun 2020 14:14
Last Modified: 04 Jun 2020 14:25
URI: http://usir.salford.ac.uk/id/eprint/57167

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