Detection and identification of ethanal-derived spin-trapped free radicals using headspace thermal desorption gas chromatography-mass spectrometry (TD-GC-MS)

Manzoor, K, Mishra, S and Podmore, ID ORCID: https://orcid.org/0000-0002-6417-0151 2020, 'Detection and identification of ethanal-derived spin-trapped free radicals using headspace thermal desorption gas chromatography-mass spectrometry (TD-GC-MS)' , Free Radical Research .

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Access Information: This is an Accepted Manuscript of an article published by Taylor & Francis in Free Radical Research on 22nd October 2020, available online: http://www.tandfonline.com/10.1080/10715762.2020.1841183.

Abstract

In this study, we demonstrate a novel approach to the detection and identification of the products of spin-trapped free radicals. Hydroxyl free radicals were generated by Fenton-based chemistry in the presence of ethanal and the spin-trapping agent N-tert-butyl- -phenylnitrone (PBN). The resulting volatile compounds present in the reaction vial headspace were collected using thermal desorption (TD) and analysed by gas chromatography-mass spectrometry (GCMS). Eleven compounds were detected in the headspace, and their identification was aided by using either a fluorinated or deuterated analogue of PBN as an alternative spin trap and/or deuterated ethanal (CD 3CHO) as the secondary source of free radicals. The electron-ionisation (EI) mass spectra clearly demonstrate the “capture” of methyl radicals; two of the compounds detected were identified as containing one methyl group derived from ethanal, and four were shown to contain two methyl groups. This study demonstrates that sampling the reaction headspace using TD-GC-MS is a viable method for analysing products of free radical trapping, and potentially may be applied to a wide range of free radical systems.

Item Type: Article
Schools: Schools > School of Environment and Life Sciences > Biomedical Research Centre
Journal or Publication Title: Free Radical Research
Publisher: Taylor & Francis
ISSN: 1071-5762
Related URLs:
Funders: Kidscan
Depositing User: ID Podmore
Date Deposited: 23 Oct 2020 12:42
Last Modified: 23 Oct 2020 12:45
URI: http://usir.salford.ac.uk/id/eprint/58603

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