RNA-seq analysis of colorectal tumor-infiltrating myeloid-derived suppressor cell subsets revealed gene signatures of poor prognosis

Saleh, R, Sasidharan Nair, V, Al-Dhaheri, M, Khawar, M, Abu Nada, M, Alajez, NM and Elkord, E 2020, 'RNA-seq analysis of colorectal tumor-infiltrating myeloid-derived suppressor cell subsets revealed gene signatures of poor prognosis' , Frontiers in Oncology, 10 , p. 604906.

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Abstract

Elevated levels of myeloid-derived suppressor cells (MDSCs), including polymorphonuclear MDSCs (PMN-MDSCs) and immature MDSCs (I-MDSCs), are usually associated with disease progression in cancer patients, including colorectal cancer (CRC). However, biological mechanisms and molecular pathways regulated by MDSC subpopulations in the CRC tumor microenvironment (TME) have not been fully investigated. In this study, we performed transcriptomic analysis of tumor-infiltrating I-MDSCs and PMN-MDSCs isolated from tumor tissues of six CRC patients, compared to antigen-presenting cells (APCs). We also compared the transcriptomic profiles of tumor-infiltrating PMN-MDSCs to I-MDSCs. Our results showed different molecular pathways regulated by each MDSC subset, potentially reflecting their phenotypical/molecular/functional characteristics in the CRC TME. Moreover, we identified gene signatures in PMN-MDSC and I-MDSC of poor overall survival (OS) and disease-free survival (DFS) using the Cancer Genome Atlas (TCGA) dataset from patients with colon adenocarcinoma (COAD). However, functional studies are required to validate these findings.

Item Type: Article
Contributors: Karagiannis, GS (Editor), Guo, ZS (Reviewer) and Alban, T (Reviewer)
Additional Information: ** From Frontiers via Jisc Publications Router ** Licence for this article: http://creativecommons.org/licenses/by/4.0/ **Journal IDs: eissn 2234-943X **History: published_online 10-11-2020; accepted 15-10-2020; submitted 10-09-2020; collection 2020
Schools: Schools > School of Environment and Life Sciences > Biomedical Research Centre
Journal or Publication Title: Frontiers in Oncology
Publisher: Frontiers Media S.A.
ISSN: 2234-943X
Related URLs:
Funders: Qatar Biomedical Research Institute, Qatar Foundation
SWORD Depositor: Publications Router
Depositing User: Publications Router
Date Deposited: 01 Dec 2020 09:34
Last Modified: 28 Aug 2021 11:23
URI: http://usir.salford.ac.uk/id/eprint/58986

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