Differential gene expression of tumor-infiltrating CD33 + myeloid cells in advanced- versus early-stage colorectal cancer

Toor, SM, Taha, RZ, Sasidharan Nair, V, Saleh, R, Murshed, K, Abu Nada, M and Elkord, E ORCID: https://orcid.org/0000-0002-3868-0318 2021, 'Differential gene expression of tumor-infiltrating CD33 + myeloid cells in advanced- versus early-stage colorectal cancer' , Cancer Immunology, Immunotherapy, 70 (3) , pp. 803-815.

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Abstract

Abstract: Colorectal cancer (CRC) has high mortality rates, especially in patients with advanced disease stages, who often do not respond to therapy. The cellular components of the tumor microenvironment are essentially responsible for dictating disease progression and response to therapy. Expansion of different myeloid cell subsets in CRC tumors has been reported previously. However, tumor-infiltrating myeloid cells have both pro- and anti-tumor roles in disease progression. In this study, we performed transcriptomic profiling of cells of myeloid lineage (CD33+) from bulk CRC tumors at varying disease stages. We identified differentially expressed genes and pathways between CRC patients with advanced stage and early stages. We found that pro-angiogenic and hypoxia-related genes were upregulated, while genes related to immune and inflammatory responses were downregulated in CD33+ myeloid cells from patients with advanced stages, implying that immune cell recruitment and activation could be compromised in advanced disease stages. Moreover, we identified a unique “poor prognosis CD33+ gene signature” by aligning top upregulated and downregulated genes in tumor-infiltrating myeloid cells from our analyses with data from The Cancer Genome Atlas. Our results showed that this gene signature is an independent prognostic indicator for disease-specific survival in CRC patients, potentially reflecting its clinical importance.

Item Type: Article
Additional Information: ** From Springer Nature via Jisc Publications Router ** Licence for this article: http://creativecommons.org/licenses/by/4.0/ **Journal IDs: pissn 0340-7004; eissn 1432-0851 **Article IDs: publisher-id: s00262-020-02727-0; manuscript: 2727 **History: published 03-2021; published 30-09-2020; online 30-09-2020; registration 13-09-2020; accepted 13-09-2020; submitted 14-07-2020
Schools: Schools > School of Environment and Life Sciences
Journal or Publication Title: Cancer Immunology, Immunotherapy
Publisher: Springer
ISSN: 0340-7004
Related URLs:
Funders: Qatar Foundation, Qatar Biomedical Research Institute
SWORD Depositor: Publications Router
Depositing User: Publications Router
Date Deposited: 01 Mar 2021 09:07
Last Modified: 01 Mar 2021 09:15
URI: http://usir.salford.ac.uk/id/eprint/59703

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