In depth characterisation of the biomolecular coronas of polymer coated inorganic nanoparticles with differential centrifugal sedimentation

Perez-Potti, A, Lopez, H, Pelaz, B, Abdelmonem, A, Soliman, MG, Schoen, I, Kelly, PM, Dawson, KA, Parak, WJ, Krpetic, Z ORCID: https://orcid.org/0000-0003-1129-6831 and Monopoli, MP 2021, 'In depth characterisation of the biomolecular coronas of polymer coated inorganic nanoparticles with differential centrifugal sedimentation' , Scientific Reports, 11 (1) , p. 6443.

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Abstract

Advances in nanofabrication methods have enabled the tailoring of new strategies towards the controlled production of nanoparticles with attractive applications in healthcare. In many cases, their characterisation remains a big challenge, particularly for small-sized functional nanoparticles of 5 nm diameter or smaller, where current particle sizing techniques struggle to provide the required sensitivity and accuracy. There is a clear need for the development of new reliable characterisation approaches for the physico-chemical characterisation of nanoparticles with significant accuracy, particularly for the analysis of the particles in the presence of complex biological fluids. Herein, we show that the Differential Centrifugal Sedimentation can be utilised as a high-precision tool for the reliable characterisation of functional nanoparticles of different materials. We report a method to correlate the sedimentation shift with the polymer and biomolecule adsorption on the nanoparticle surface, validating the developed core–shell model. We also highlight its limit when measuring nanoparticles of smaller size and the need to use several complementary methods when characterising nanoparticle corona complexes.

Item Type: Article
Schools: Schools > School of Environment and Life Sciences > Biomedical Research Centre
Journal or Publication Title: Scientific Reports
Publisher: Nature Publishing Group
ISSN: 2045-2322
Related URLs:
Funders: European Commission, European Commission, European Commission, Deutsche Forschungsgemeinschaf (DFG), European Commission, Kidscan, Irish Research Council
Depositing User: Dr Zeljka Krpetic
Date Deposited: 22 Mar 2021 08:31
Last Modified: 28 Aug 2021 10:57
URI: http://usir.salford.ac.uk/id/eprint/59899

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