Analysis of fixed and live single cells using optical photothermal infrared with concomitant raman spectroscopy

Spadea, A, Denbigh, JL ORCID: https://orcid.org/0000-0002-6616-7757, Lawrence, MJ, Kansiz, M and Gardner, P ORCID: https://orcid.org/0000-0003-4034-3764 2021, 'Analysis of fixed and live single cells using optical photothermal infrared with concomitant raman spectroscopy' , Analytical Chemistry, 93 (8) , pp. 3938-3950.

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Abstract

This paper reports the first use of a novel completely optically based photothermal method (O-PTIR) for obtaining infrared spectra of both fixed and living cells using a quantum cascade laser (QCL) and optical parametric oscillator (OPO) laser as excitation sources, thus enabling all biologically relevant vibrations to be analyzed at submicron spatial resolution. In addition, infrared data acquisition is combined with concomitant Raman spectra from exactly the same excitation location, meaning the full vibrational profile of the cell can be obtained. The pancreatic cancer cell line MIA PaCa-2 and the breast cancer cell line MDA-MB-231 are used as model cells to demonstrate the capabilities of the new instrumentation. These combined modalities can be used to analyze subcellular structures in both fixed and, more importantly, live cells under aqueous conditions. We show that the protein secondary structure and lipid-rich bodies can be identified on the submicron scale.

Item Type: Article
Additional Information: ** Article version: VoR ** From Crossref journal articles via Jisc Publications Router ** Licence for VoR version of this article starting on 17-02-2021: https://creativecommons.org/licenses/by/4.0/ **Journal IDs: pissn 0003-2700; eissn 1520-6882 **History: published 02-03-2021; issued 17-02-2021; published_online 17-02-2021
Schools: Schools > School of Environment and Life Sciences
Journal or Publication Title: Analytical Chemistry
Publisher: American Chemical Society (ACS)
ISSN: 0003-2700
Related URLs:
Funders: AstraZeneca, University of Manchester, Division of Pharmacy and Optometry, University of Manchester
SWORD Depositor: Publications Router
Depositing User: Publications Router
Date Deposited: 04 May 2021 12:43
Last Modified: 28 Aug 2021 10:48
URI: http://usir.salford.ac.uk/id/eprint/60149

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