The relationship between the infection of Toxoplasma gondii and Alzheimer’s disease

Alawfi, B 2021, The relationship between the infection of Toxoplasma gondii and Alzheimer’s disease , PhD thesis, University of Salford.

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Abstract

Toxoplasma gondii is a zoonotic parasite that commonly causes infections in a variety of warm-blooded animals including humans. This parasite causes a disease referred to as toxoplasmosis. T. gondii infections are known to have a significant impact on the host brain since the parasite can penetrate the blood-brain barrier and extensively colonize the central nervous system (CNS) for an extended period. Attacks of the brain tissue by the parasite can cause both neurological and structural damage. The parasite infects the brain, forms a cyst and affects several parts of the brain, including the amygdala, hippocampus, other cortical regions, and cerebellum; this can result in alterations in the hosts' behaviour. Studies have proposed that infections caused by T. gondii pose a risk in the development of several neuropsychiatric disorders like Alzheimer’s disease, Schizophrenia, and Parkinson's disease. The aims of this study were to conduct a pilot study on the association between T. gondii infections and Alzheimer's disease using tissue samples from the Manchester Brain Bank (MBB) and to investigate the effect of the parasite on oxidative stress in cultured human brain cells As a pilot study, previously prepared slides from 124 brain sections from Alzheimer’s patients and controls were examined for the presence of Toxoplasma gondii cysts. Despite a 10% prevalence of T. gondii in humans in the UK, no cysts were detected in any of the sections. It was concluded that unsustainably large tissue samples would be required from MBB to pursue an investigation using this approach. Instead, a line of investigation was pursued that explored the interactions between T. gondii infections and oxidative stress (a feature of neurological diseases such as Alzheimer's) using the development of a model system. SH-SY5Y cells were used as culture models for neuronal cells and flow cytometry and MTT assays were conducted to determine cell viability. To evaluate the effects of oxidative stress, differentiated and undifferentiated SH-SY5Y cells, were examined for cell viability and the effects of infection by T. gondii type I and II were investigated. When high doses of H2O2 [250 and 500uM] were used for 24 hours in undifferentiated SH-SY5Y cells, there was a reduction in the cell viability to 8.66% and 4.66% respectively. Conversely, when SH-SY5Y cells (differentiated) were subjected to high doses of H2O2 (250 and 500

Item Type:	Thesis (PhD)
Contributors:	Hide, G (Supervisor)
Schools:	Schools > School of Environment and Life Sciences > Biomedical Research Centre
Depositing User:	Bader Alawfi
Date Deposited:	09 Jun 2021 12:43
Last Modified:	27 Aug 2021 21:52
URI:	https://usir.salford.ac.uk/id/eprint/60184

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