A novel approach to the analysis of spin-trapped free radicals using dimethyl sulfoxide and Gas Chromatography – Mass Spectrometry (GC-MS) with both solvent extraction and headspace solid phase microextraction (HS-SPME)

Mishra, SS, Manzoor, K, Zafar, M and Podmore, ID ORCID: https://orcid.org/0000-0002-6417-0151 2021, 'A novel approach to the analysis of spin-trapped free radicals using dimethyl sulfoxide and Gas Chromatography – Mass Spectrometry (GC-MS) with both solvent extraction and headspace solid phase microextraction (HS-SPME)' , Free Radical Research .

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Abstract

In this study, we have utilized a novel strategy based upon the use of dimethyl sulfoxide (DMSO) and gas chromatography-mass spectrometry (GC-MS)for the detectionand identification ofspin-trapped free radicals. Hydroxymethyl (.CH2OH) radicals, generated by Fenton-type chemistry,have been trapped byN-tert-butyl-α-phenylnitrone (PBN) or one of its derivatives in the presence of DMSOto form a1,3-diadduct[PBN-(CH2OH)(CH3)],which may be detected directly in the reaction mixture following chloroform extraction orin the reaction vial headspace bysampling with SPME. Separation and identification have been carried out by capillary gas chromatography coupled to electron-ionization mass spectrometry (EI-MS). The results demonstrate that using DMSO aidsGC-MS analysis of spin-trapped free radicals via the formation ofradical-methyl di-adducts that are sufficiently volatile to be sampled both in the headspace or by an extracting solvent without the need for a derivatization step using silylating agents.

Item Type: Article
Schools: Schools > School of Environment and Life Sciences > Biomedical Research Centre
Journal or Publication Title: Free Radical Research
Publisher: Taylor & Francis
ISSN: 1071-5762
Related URLs:
Funders: Kidscan
Depositing User: ID Podmore
Date Deposited: 27 Sep 2021 09:08
Last Modified: 01 Oct 2021 13:45
URI: http://usir.salford.ac.uk/id/eprint/61924

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