High resistance to Toxoplasma gondii infection in inducible nitric oxide synthase (iNOS) knockout rats

Wang, Z-J, Yu, S-M, Gao, J-M, Zhang, P, Hide, G ORCID: https://orcid.org/0000-0002-3608-0175, Yamamoto, M, Lai, D-H ORCID: https://orcid.org/0000-0002-4709-1507 and Lun, Z-R 2021, 'High resistance to Toxoplasma gondii infection in inducible nitric oxide synthase (iNOS) knockout rats' , iScience, 24 (11) , p. 103280.

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Abstract

Nitric oxide (NO) is an important immune molecule which acts against extra- and intracellular pathogens in most hosts. However, after knockout of inducible nitric oxide synthase (iNOS-/-) in Sprague Dawley (SD) rats, these iNOS-/- rats were found to be completely resistant to Toxoplasma gondii infection. Once the iNOS-/- rat peritoneal macrophages (PM) were infected with T. gondii, they produced high levels of reactive oxygen species (ROS) triggered by GRA43 secreted by T. gondii, which damaged the parasitophorous vacuole membrane and PM mitochondrial membranes within a few hours post infection. Further evidence indicated that the high levels of ROS caused mitochondrial superoxide dismutase 2 depletion and induced PM pyroptosis and cell death. This discovery of complete resistance to T. gondii infection, in the iNOS-/--SD rat, demonstrates a strong link between NO and ROS in immunity to T. gondii infection and showcases a potentially novel and effective backup innate immunity system.

Item Type:	Article
Additional Information:	** Article version: AM ** From Elsevier via Jisc Publications Router ** Licence for AM version of this article starting on 14-10-2021: http://creativecommons.org/licenses/by-nc-nd/4.0/ **Journal IDs: issn 25890042 **History: issued 15-10-2021; accepted 13-10-2021
Schools:	Schools > School of Environment and Life Sciences > Biomedical Research Centre
Journal or Publication Title:	iScience
Publisher:	Elsevier/Cell Press
ISSN:	2589-0042
Related URLs:	Publication
Funders:	National Key R&D Program of China, National Natural Science Foundation of China, University of Salford
SWORD Depositor:	Publications Router
Depositing User:	Publications Router
Date Deposited:	18 Oct 2021 09:05
Last Modified:	15 Feb 2022 17:11
URI:	https://usir.salford.ac.uk/id/eprint/62079

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