Circulating and tumor-infiltrating immune checkpoint-xepressing CD8+ Treg/T Cell subsets and their associations with disease-free survival in colorectal cancer patients

Alsalman, A, Al-Mterin, MA ORCID: https://orcid.org/0000-0003-1245-8118, Murshed, K ORCID: https://orcid.org/0000-0001-8045-363X, Alloush, F, Al-Shouli, ST ORCID: https://orcid.org/0000-0002-3473-0883, Toor, SM ORCID: https://orcid.org/0000-0002-4683-3329 and Elkord, E ORCID: https://orcid.org/0000-0002-3868-0318 2022, 'Circulating and tumor-infiltrating immune checkpoint-xepressing CD8+ Treg/T Cell subsets and their associations with disease-free survival in colorectal cancer patients' , Cancers, 14 (13) , p. 3194.

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Abstract

T cells in the tumor microenvironment (TME) have diverse roles in anti-tumor immunity, including orchestration of immune responses and anti-tumor cytotoxic attack. However, different T cell subsets may have opposing roles in tumor progression, especially in inflammation-related cancers such as colorectal cancer (CRC). In this study, we phenotypically characterized CD3+CD4- (CD8+) T cells in colorectal tumor tissues (TT), normal colon tissues (NT) and in circulation of CRC patients. We investigated the expression levels of key immune checkpoints (ICs) and Treg-related markers in CD8+ T cells. Importantly, we investigated associations between different tumor-infiltrating CD8+ T cell subpopulations and disease-free survival (DFS) in CRC patients. We found that FoxP3 expression and ICs including PD-1, CTLA-4, TIM-3, and LAG-3 were significantly increased in tumor-infiltrating CD8+ T cells compared with NT and peripheral blood. In the TME, we found that TIM-3 expression was significantly increased in patients with early stages and absent lymphovascular invasion (LVI) compared to patients with advanced stages and LVI. Importantly, we report that high levels of certain circulating CD8+ T cell subsets (TIM-3-expressing, FoxP3-Helios-TIM-3+ and FoxP3-Helios+TIM-3+ cells) in CRC patients were associated with better DFS. Moreover, in the TME, we report that elevated levels of CD25+ and TIM-3+ T cells, and FoxP3+Helios-TIM-3+ Tregs were associated with better DFS.

Item Type: Article
Schools: Schools > School of Health and Society
Journal or Publication Title: Cancers
Publisher: MDPI
ISSN: 2072-6694
Related URLs:
SWORD Depositor: Publications Router
Depositing User: Publications Router
Date Deposited: 06 Sep 2022 10:30
Last Modified: 06 Sep 2022 10:30
URI: http://usir.salford.ac.uk/id/eprint/64535

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