Perivascular adipose tissue anticontractile function is mediated by both endothelial and neuronal nitric oxide synthase isoforms.

Saxton, S, Withers, SB ORCID: https://orcid.org/0000-0002-7021-881X and Heagerty, AM 2022, 'Perivascular adipose tissue anticontractile function is mediated by both endothelial and neuronal nitric oxide synthase isoforms.' , Journal of Vascular Research, 59 (5) , pp. 1-15.

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Abstract

The mechanism of the perivascular adipose tissue (PVAT) anticontractile effect is well characterized in rodent visceral vascular beds; however, little is known about the mechanism of PVAT anticontractile function in subcutaneous vessels. In addition, we have previously shown that PVAT anticontractile function is nitric oxide synthase (NOS) dependent but have not investigated the roles of NOS isoforms. Here, we examined PVAT anticontractile function in the mouse gracilis artery, a subcutaneous fat depot, in lean control and obese mice and investigated the mechanism in comparison to a visceral depot. Using the wire myograph, we generated responses to noradrenaline and electrical field stimulation in the presence of pharmacological tools targeting components of the known PVAT anticontractile mechanism. In addition, we performed ex vivo "fat transplants" in the organ bath. The mechanism of PVAT anticontractile function is similar between subcutaneous and visceral PVAT depots. Both endothelial and neuronal NOS isoforms mediated the PVAT anticontractile effect. Loss of PVAT anticontractile function in obesity is independent of impaired vasoreactivity, and function can be restored in visceral PVAT by NOS activation. Targeting NOS isoforms may be useful in restoring PVAT anticontractile function in obesity, ameliorating increased vascular tone, and disease. [Abstract copyright: © 2022 The Author(s). Published by S. Karger AG, Basel.]

Item Type: Article
Schools: Schools > School of Environment and Life Sciences
Journal or Publication Title: Journal of Vascular Research
Publisher: Karger
ISSN: 1018-1172
SWORD Depositor: Publications Router
Depositing User: Publications Router
Date Deposited: 09 Sep 2022 09:45
Last Modified: 09 Sep 2022 09:45
URI: https://usir.salford.ac.uk/id/eprint/64637

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