Anticancer phototherapy using activation of E-combretastatins by two-photon–induced isomerization

Hadfield, JA ORCID:, Bisby, RH, Scherer, KM, Botchway, SW and Parker, AW 2014, 'Anticancer phototherapy using activation of E-combretastatins by two-photon–induced isomerization' , Journal of Biomedical Optics, 20 (5) , pp. 1004-1.

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Access Information: Copyright (2014) Society of Photo-Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.


The photoisomerization of relatively nontoxic E-combretastatins to clinically active Z-isomers is shown to occur in solution through both one- and two-photon excitations at 340 and 625 nm, respectively. The photoisomerization is also demonstrated to induce mammalian cell death by a two-photon absorption process at 625 nm. Unlike conventional photodynamic therapy (PDT), the mechanism of photoisomerization is oxygen- independent and active in hypoxic environments such as in tumors. The use of red or near-infrared (NIR) light for two-photon excitation allows greater tissue penetration than conventional UV one-photon excitation. The results provide a baseline for the development of a novel phototherapy that overcomes nondiscriminative systemic toxicity of Z-combretastatins and the limitations of PDT drugs that require the presence of oxygen to promote their activity, with the added benefits of two-photon red or NIR excitation for deeper tissue penetration.

Item Type: Article
Themes: Health and Wellbeing
Schools: Schools > School of Environment and Life Sciences > Biomedical Research Centre
Journal or Publication Title: Journal of Biomedical Optics
Publisher: Society of Photo-optical Instrumentation Engineers (SPIE)
Refereed: Yes
ISSN: 1083-3668
Related URLs:
Funders: Rutherford Laboratories
Depositing User: JA Hadfield
Date Deposited: 31 Oct 2014 16:57
Last Modified: 15 Feb 2022 18:47

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