Biology and taxonomy of crAss-like Bacteriophages, the most abundant virus in the human gut

Guerin, E, Shkoporov, A, Stockdale, SR, Clooney, AG, Ryan, FJ, Sutton, TDS, Draper, LA, Gonzalez Tortuero, E ORCID:, Paul Ross, R and Hill, C 2018, 'Biology and taxonomy of crAss-like Bacteriophages, the most abundant virus in the human gut' , Cell Host & Microbe, 24 (5) , 653-664.e6.

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CrAssphages represent the most abundant virus in the human gut microbiota, but the lack of available genome sequences for comparison has kept them enigmatic. Recently, sequence-based classification of distantly related crAss-like phages from multiple environments was reported, leading to a proposed familial-level taxonomic group. Here, we assembled the metagenomic sequencing reads from 702 human fecal virome/phageome samples and analyzed 99 complete circular crAss-like phage genomes and 150 contigs ≥70 kb. In silico comparative genomics and taxonomic analysis enabled a classification scheme of crAss-like phages from human fecal microbiomes into four candidate subfamilies composed of ten candidate genera. Laboratory analysis was performed on fecal samples from an individual harboring seven distinct crAss-like phages. We achieved crAss-like phage propagation in ex vivo human fecal fermentations and visualized short-tailed podoviruses by electron microscopy. Mass spectrometry of a crAss-like phage capsid protein could be linked to metagenomic sequencing data, confirming crAss-like phage structural annotations.

Item Type: Article
Schools: Schools > School of Environment and Life Sciences
Journal or Publication Title: Cell Host & Microbe
Publisher: Elsevier/Cell Press
ISSN: 1931-3128
Related URLs:
Funders: Science Foundation Ireland (SFI), Janssen Biotech
Depositing User: E Gonzalez Tortuero
Date Deposited: 22 Feb 2021 11:49
Last Modified: 27 Aug 2021 21:50

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