Guerin, E, Shkoporov, A, Stockdale, SR, Clooney, AG, Ryan, FJ, Sutton, TDS, Draper, LA, Gonzalez Tortuero, E 
ORCID: https://orcid.org/0000-0001-8934-6822, Paul Ross, R and Hill, C
2018,
'Biology and taxonomy of crAss-like Bacteriophages, the most abundant virus in the human gut'
, Cell Host & Microbe, 24 (5)
, 653-664.e6.
Abstract
CrAssphages represent the most abundant virus in the human gut microbiota, but the lack of available genome sequences for comparison has kept them enigmatic. Recently, sequence-based classification of distantly related crAss-like phages from multiple environments was reported, leading to a proposed familial-level taxonomic group. Here, we assembled the metagenomic sequencing reads from 702 human fecal virome/phageome samples and analyzed 99 complete circular crAss-like phage genomes and 150 contigs ≥70 kb. In silico comparative genomics and taxonomic analysis enabled a classification scheme of crAss-like phages from human fecal microbiomes into four candidate subfamilies composed of ten candidate genera. Laboratory analysis was performed on fecal samples from an individual harboring seven distinct crAss-like phages. We achieved crAss-like phage propagation in ex vivo human fecal fermentations and visualized short-tailed podoviruses by electron microscopy. Mass spectrometry of a crAss-like phage capsid protein could be linked to metagenomic sequencing data, confirming crAss-like phage structural annotations.
| Item Type: | Article |
|---|---|
| Schools: | Schools > School of Environment and Life Sciences |
| Journal or Publication Title: | Cell Host & Microbe |
| Publisher: | Elsevier/Cell Press |
| ISSN: | 1931-3128 |
| Related URLs: | |
| Funders: | Science Foundation Ireland (SFI), Janssen Biotech |
| Depositing User: | E Gonzalez Tortuero |
| Date Deposited: | 22 Feb 2021 11:49 |
| Last Modified: | 27 Aug 2021 21:50 |
| URI: | https://usir.salford.ac.uk/id/eprint/59632 |
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