Sayan, AE, Roperch, JP, Sayan, BS 
ORCID: https://orcid.org/0000-0003-2305-1970, Rossi, M, Pinkoski, MJ, Knight, RA, Willis, AE and Melino, G
2007,
'Generation of ΔTAp73 proteins by translation from a putative internal ribosome entry site'
, Annals of the New York Academy of Sciences, 1095 (1)
, pp. 315-324.
Abstract
p73 belongs to a family of transcription factors, including p53 and p63, that mediate response to DNA damage and cellular stress by inducing DNA repair, cell cycle arrest, and apoptosis. TP73 gene contains two promotors and several splice variants resulting in up to 24 possible permutations of p73 proteins which underlies the complexity of the family and its regulatory mechanisms. p73 variants lacking the N-terminal, denoted as ΔTAp73, are not transcriptionally competent and they act in a dominant negative fashion over TAp73. ΔTAp73 isoforms can be generated by alternative promotor usage, giving rise to ΔNp73, or alternative splicing of exons 2, 3 or 2, and 3 together. Such transcript isoforms potentially produce oncogenic proteins and they were shown to be present in primary tumors and tumor-derived cell lines. We investigated the possibility of additional mechanisms by which p73 protein could be regulated and discovered a putative internal ribosome entry site (IRES) in exon 2. Translation initiation of TAp73 mRNA results in a ΔNp73-like peptide, thus demonstrating an additional mechanism whereby a ΔTA p73 protein is produced from a transcript originally generated from the P1 promotor of the p73 gene.
| Item Type: | Article |
|---|---|
| Schools: | Schools > School of Environment and Life Sciences |
| Journal or Publication Title: | Annals of the New York Academy of Sciences |
| Publisher: | Wiley |
| ISSN: | 0077-8923 |
| Depositing User: | BS Sayan |
| Date Deposited: | 06 Feb 2023 15:30 |
| Last Modified: | 06 Feb 2023 15:30 |
| URI: | https://usir.salford.ac.uk/id/eprint/66350 |
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