NAPO as a novel marker for apoptosis

Sayan, BS ORCID: https://orcid.org/0000-0003-2305-1970, Ince, G, Sayan, AE and Ozturk, M 2001, 'NAPO as a novel marker for apoptosis' , Journal of Cell Biology, 155 (5) , pp. 719-724.

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Abstract

Apoptosis or programmed cell death plays a pivotal role in embryonic development and maintenance of homeostasis. It is also involved in the etiology of pathophysiological conditions such as cancer, neurodegenerative, autoimmune, infectious, and heart diseases. Consequently, the study of apoptosis is now at center of both basic and clinical research applications. Therefore, sensitive and simple apoptosis detection techniques are required. Here we describe a monoclonal antibody–defined novel antigen, namely NAPO (negative in apoptosis), which is specifically lost during apoptosis. The anti-NAPO antibody recognizes two nuclear polypeptides of 60 and 70 kD. The antigen is maintained in quiescent and senescent cells, as well as in different phases of the cell cycle, including mitosis. Thus, immunodetection of NAPO antigen provides a specific, sensitive, and easy method for differential identification of apoptotic and nonapoptotic cells.

Item Type:	Article
Schools:	Schools > School of Environment and Life Sciences
Journal or Publication Title:	Journal of Cell Biology
Publisher:	Rockefeller University Press
ISSN:	0021-9525
Depositing User:	BS Sayan
Date Deposited:	06 Feb 2023 15:08
Last Modified:	06 Feb 2023 15:15
URI:	https://usir.salford.ac.uk/id/eprint/66356

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