Electrochemical detection of asparaginase antibodies using bifunctionalized carbon interfaces

Marshall, D and Lehr, J ORCID: https://orcid.org/0000-0001-5698-7244 2023, 'Electrochemical detection of asparaginase antibodies using bifunctionalized carbon interfaces' , Electroanalysis .

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Abstract

The early detection of anti-asparaginase biomarker can facilitate timely modification of asparaginase chemotherapy, thereby avoiding serious complications. Herein we describe the preparation of a novel electrochemical biosensing interface for rapid detection of anti-asparaginase in the picomolar range (1–10000 pM). Coimmobilization of ferrocene and asparaginase on a carbon interface (via diazonium grafting) facilitates transduction through attenuation of the surface-bound ferrocene redox couple. The limit of detection of 0.8 pM for this point-of-care applicable method compares favourably to that of traditional faradaic assaying (2.0 pM) where transduction occurs by the target blocking the diffusion of the solution redox probe [Fe(CN)6]3-/4-.

Item Type:	Article
Schools:	Schools > School of Environment and Life Sciences > Biomedical Research Centre
Journal or Publication Title:	Electroanalysis
Publisher:	Wiley
ISSN:	1040-0397
Funders:	Kidscan Cancer Research Charity, UK Research & Innovation (UKRI), Engineering & Physical Sciences Research Council (EPSRC)
Depositing User:	J Lehr
Date Deposited:	13 Feb 2023 14:42
Last Modified:	24 Mar 2023 07:27
URI:	https://usir.salford.ac.uk/id/eprint/66423

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